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Description

L-BrothLuria Broth Base (Miller's Modification) Luria Broth Base or L Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E. coli cells in molecular biology. L Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient

3 KH2PO4 6

its gelling

protect against light

Component g/l Peptone 14 Yeast Extract 7 KH2PO4 0

The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes

Eur and USP Heavy metals <5 ppm Ferrocyanides <0

Dry Base) ≥99

15 grams of powdered medium in 1 litre distilled water

coli cells

These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain

Peptones contain a mix of peptides

02 Cyanocobalamin 0